Effect of Extraction Solvent and Harvest Time on Recovery of Bioactive Com-pounds, Antioxidant and Cancer Cell Growth Inhibition Activities of Korean Ca-mellia mistletoe

This study aimed to determine the phytochemical profiles,in vitroantioxidant and antiproliferative properties of KoreanCamellia mistletoe(Korthalsella japon-ica(Thunb.) Engl.) depending on harvest time (August November) sol-vent (100% methanol, 70% ethanol, hot water). TheCamellia japonicaL. mistle-toe extracts were analyzed for total phenol, flavonoid, carotenoid, L-ascorbic acid contents antioxidant such as scavenging capacities (1,1-diphenyl-2-pic-rylhydrazyl (DPPH) NO•),ferrous ion chelating reducing power. Concur-rently, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) as-say was used assess against human cancer cell lines; MCF (human breast cells), HeLa cervical A375 malignant melanoma HCT116 colon HepG2 liver cells) A549 non-small lung adenocarcinoma cells). The results showed that extraction solvent had significant impacts anticancer activities selectivity free phenolic compounds inCamellia ja-ponicaL. mistletoe. Among all tested extracts, highest amounts phe-nolic flavonoids content found in ethanol ofCamellia japon-icaL. mistletoe harvested November, while methanol collected August carote-noids acidscompared other extracts. Additionally, NO•radical activity whereas strongest DPPH radical ofCamel-lia November. Furthermore, much higher cells than eth-anol In conclusion, dependence type time. With optimum solvent, they boast a wide range promising medical, pharmaceutical, food applications